Pharmacodynamics Test of Soy Beverage

Containing Royal Jelly

 

Abstract:

Malnourished pure line ICR mice were fed intragastrically (i. g.) with Soy Beverage Containing Royal Jelly (SBRJ) at the dose of 2.6-6g.kh-1 (SBRJ) for 15 days. It showed that SBRJ could promote mice growth, physical strength and durability of the hearts to anoxia apparently. When the dose of 4-6g.kg-1 was used for 30 days. The level of LPO in serum decreased and activity of SOD in RBC increased significantly. In addition, the ratio of SOD/LPO increased. Taking in SBRJ could reduce the high level of SGPT induced by thioacetamide (TAA). The results indicated the formulation have the function of liver protection. Taking in high dose (6g.kg-1) SBRJ could relief the mice from leukopenia induced by cyclophosphamide (CTX). The WBC has increased.

Soy beverage containing Royal Jelly (SBRJ) is made of royal jelly, soybean, and yeast 851 though bio-technical fermented processing. As a rich nutritional beverage, it can significantly improve health condition. Our study investigated the effect of SBRJ on improving immune response, preventing malnutrition reducing side effects, and treating malfunction of liver.

1. Trial Drug

SBJR are supplied by manufacture. Product code: # 920223.

2. Trial Mouse

Both ICR pure line mice, 320pcs, weighing 13-24g, and SD pure line mice, 80pcs, weighing 120-140g, are supplied by Shanghai Shipper-BK Experimental Animal Co.

3. Method and Result

3.1 The effect of SBRJ on mice growth and physical duration

0 ICR pure line male mice were randomly grouped into 8 groups. Group one was fed with mouse food as positive control group. Group two was fed with rabbit food as negative control group. Group 3-8 was experimental groups fed with rabbit food together with SBJR.

The weight of mice was measured daily for 15 consecutive days. The result shows that the growth of mice fed with rabbit food was retarded (p<0.001) while intragastrically administration of SBRJ diminished remarkable growth retardation caused by rabbit food. After 15days, mice were anathaetized by ether and their trachea were separated and ligated with thread. The disappearing time of QRS wave was recorded using cardioelectrography. The result is shown following:

As indicated by disappearing time of QRS wave, SBRJ significantly increased the durability mouse heart to anoxia (p<0.001).

3.2 The effect of SBRJ on the physical strength of mouse.

80 ICR pure line mice were randomly grouped into 8 groups. Group one was fed with mouse food as positive control group. Group two was fed with rabbit food as negative control group. Group 3-8 was experimental groups fed with rabbit food together with SBRJ. Mice were fed once a day for two consecutive weeks. Half-hour after the last feeding, the tail of mouse was tied with a fuse weighting 10% of mouse body weight. The mice were then let swim in a 22±°C water pool. Start the timer once the mice get to the pool and end the timer when the mice sank to the bottom on the pool. The time was recorded to evaluate the physical strength of mice.

The result indicates that long time feeding with rabbit food reduces the physical strength of mice (p<0.05) while supplement of SBRJ remarkably reverse the effect of rabbit feeding. The group supplemented with high dose SBRJ has even better physical strength than positive control group (p<0.05).

3.3 The effect on serum LPO

80 SD pure line male mice were randomly grouped into 8 groups. They were fed once a day for one month as shown in table 5. One hour after last feeding, the mice were decapitated and blood was sampled. SOD activity of RBC was detected. While serum LPO level was measured with TBA spectrometer.

The result shows that both Vt. E and SBRJ remarkably reduced mouse serum LPO level and increased SOD activity of RBC. The increase of SOD/LPO ratio indicates that SBRJ could help to better eliminate LPO.

3.4 The effect on liver protection:

80 ICR pure line male mice were randomly grouped into 8 groups. Stop feeding them for at least 8hrs before experiment starts. Group 4-8 was fed intragastrically with SBRJ as shown in table 6. Group 3 was intraparentally injected with 80mg/. At day 8, all mice except group one was intraparentlly injected with 80mg/kg TAA to toxicate liver. 24 hours after toxification, mouse blood was sample by eyeball. Serum SGPT level was measured as shown in OD value.

The result shows that SBRJ can dramatically protects liver.

3.5 The effects on relieving mouse's leukopenia induced by yclophosphamide (CTX).

80 female ICR pure line mice were randomly grouped into 8 groups. They were explained with hepacarcinomas cell (HAC) and fed with test drugs as shown in table 7. 24 hrs after last feeding, tails of mice were cut and the blood was sampled. WBC was counted.

The result shows that high dose SBRJ can significantly block the WBC reduction induced by CTX in HAC explanted mouse.

Discussion:

SBRJ contains high level of protein, amino acid, polysaccharide, vitamins and essential elements. Our study shows that SBRJ could promote growth, physical strength and durability of the hearts to anoxia. They also decrease serum LPO level and increase the activity